Extended Data Fig. 7. Targeting the NOX feedback loop by chemical inhibitors.
a, NOX4 is the dominant NOX isoform in RPE-1 cells. RT-qPCR was performed to quantify the relative mRNA levels of NOX1-4 in RPE-1 cells. Data represent mean ± s.d. of three technical replicates. Data shown is a representative of three biological repeats. b, Small-molecule inhibitors targeting the NOX loop do not exhibit antioxidant potential. Cell-free antioxidant potentials of Trolox (32 µM), GKT137831 (5 µM), LY294002 (100 µM), and dasatinib (10 µM) were measured as the DPPH absorbance at 517 nm relative to DMSO (vehicle control). Data represent mean ± s.d. from three wells.