Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1976 Aug 15;158(2):295–305. doi: 10.1042/bj1580295

A kinetic analysis of the changes in fluorescence on the interaction of 8-anilinonaphthalene-1-sulphonate with submitochondrial particles.

N Gains, A P Dawson
PMCID: PMC1163971  PMID: 985430

Abstract

A comparison of the fluorescence change on the addition of 8-anilinonaphthalene-1-sulphonate to succinate-energized submitochondrial particles with that on the addition of succinate to submitochondrial particles incubated with 8-anilinonaphthalene-1-sulphonate shows that these changes in fluorescence may be explained solely in terms of 8-anilinonaphthalene-1-sulphonate binding. This comparison does not support the proposal of an 8-anilinonaphthalene-1-sulphonate-monitored change in the conformation of submitochondrial-particle membranes [Brocklehurst, Freedman, Hancock & Radda (1970) Biochem. J.116, 721-731]. The biphasic nature of the decrease in fluorescence, which was found to follow the addition of uncoupler to submitochondrial particles incubated with ATP or succinate, or of antimycin A to submitochondrial particles incubated with succinate, does not support the existence of 'aplectic' and 'symplectic' states of the mitochondrial membrane [Barrett-Bee & Radda (1972) Biochim, Biophys. Acta 267, 211-215].

Full text

PDF
300

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Azzi A., Chance B., Radda G. K., Lee C. P. A fluorescence probe of energy-dependent structure changes in fragmented membranes. Proc Natl Acad Sci U S A. 1969 Feb;62(2):612–619. doi: 10.1073/pnas.62.2.612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Azzi A. Redistribution of the electrical charge of the mitochondrial membrane during energy conservation. Biochem Biophys Res Commun. 1969 Oct 8;37(2):254–260. doi: 10.1016/0006-291x(69)90727-x. [DOI] [PubMed] [Google Scholar]
  3. Azzi A., Santato M. Interaction of ethidium with the mitochondrial membrane: cooperative binding and energy-linked changes. Biochem Biophys Res Commun. 1971 Jul 2;44(1):211–217. doi: 10.1016/s0006-291x(71)80180-8. [DOI] [PubMed] [Google Scholar]
  4. Azzi A., Santato M. On the interpretation of energy linked 1-anilino-8-naphthalene sulfonic acid fluorescence changes in mitochondrial fragments. FEBS Lett. 1972 Oct 15;27(1):35–38. doi: 10.1016/0014-5793(72)80403-4. [DOI] [PubMed] [Google Scholar]
  5. Barrett-Bee K., Radda G. K. On the nature of the energy-linked guantum yield change in anilino-naphthalene sulphonate fluorescence in submitochondrial particles. Biochim Biophys Acta. 1972 Apr 20;267(1):211–215. doi: 10.1016/0005-2728(72)90153-3. [DOI] [PubMed] [Google Scholar]
  6. Brocklehurst J. R., Freedman R. B., Hancock D. J., Radda G. K. Membrane studies with polarity-dependent and excimer-forming fluorescent probes. Biochem J. 1970 Feb;116(4):721–731. doi: 10.1042/bj1160721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chance B., Azzi A., Mela L., Radda G., Vainio H. Local anesthetic induced changes of a membrane-bound fluorochrome A link between ion uptake and membrane structure. FEBS Lett. 1969 Apr;3(1):10–13. doi: 10.1016/0014-5793(69)80083-9. [DOI] [PubMed] [Google Scholar]
  8. Ferguson S. J., Lloyd W. J., Radda G. K. On the nature of the energised state of submitochondrial particles; investigations with N-aryl naphthalene sulphonate probes. Biochim Biophys Acta. 1976 Feb 16;423(2):174–188. doi: 10.1016/0005-2728(76)90176-6. [DOI] [PubMed] [Google Scholar]
  9. Gains N., Dawson A. P. 8-Anilinonaphthalene-1-sulphonate interaction with whole and disrupted mitochondria: a re-evaluation of the use of double-reciprocal plots in the derivation of binding parameters for fluorescent probes binding to mitochondrial membranes. Biochem J. 1975 Apr;148(1):157–160. doi: 10.1042/bj1480157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gains N., Dawson A. P. Transmembrane electrophoresis of 8-anilino-1-naphthalenesulfonate through egg lecithin liposome membranes. J Membr Biol. 1975 Dec 4;24(3-4):237–278. doi: 10.1007/BF01868625. [DOI] [PubMed] [Google Scholar]
  11. Layton D. G., Symmons P., Williams W. P. An analysis of the binding of 8-anilino-1-naphthalene-sulphonate to sub-mitochondrial particles. FEBS Lett. 1974 Apr 15;41(1):1–7. doi: 10.1016/0014-5793(74)80939-7. [DOI] [PubMed] [Google Scholar]
  12. Racker E. Reconstitution of cytochrome oxidase vesicles and conferral of sensitivity to energy transfer inhibitors. J Membr Biol. 1972 Dec 29;10(3):221–235. doi: 10.1007/BF01867856. [DOI] [PubMed] [Google Scholar]
  13. Tson- T. Y. Transport of 8-anilino-1-naphthalenesulfonate as a probe of the effect of cholesterol on the phospholipid bilayer structures. Biochemistry. 1975 Dec 16;14(25):5415–5417. doi: 10.1021/bi00696a005. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES