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. 2024 Nov 25;14(23):3382. doi: 10.3390/ani14233382

Table 2.

List of primer sequences used for quantitative real-time PCR.

Gene Accession Number Primer Sequence (5′-3′) Size (bp)
GAPDH NM_001206359.1 F: GCTTGTCATCAATGGAAAGG 86
R: CATACGTAGCACCAGCATCA
Occludin NM_001163647.2 F: TCAGGTGCACCCTCCAGATT 112
R: TGGACTTTCAAGAGGCCTGG
Claudin-1 NM_001244539.1 F: CCTCAATACAGGAGGGAAGC 76
R: CTCTCCCCACATTCGAGATGATT
ZO-1 CV870309 F: CGATCACTCCAGCATACAAT 111
R: CACTTGGCAGAAGATTGTGA