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. 2024 Dec 9;19:13253–13265. doi: 10.2147/IJN.S490667

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© 2024 Lee et al.

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Confocal Laser Microscopy (CLSM) of the in situ zymography of the group with different treatment solutions after incubation for 24 h, observed at 20X magnification. Green fluorescence represents collagenolytic activity originating from quenched FITC-conjugated collagen breakdown by MMP. Deionized water and ethanol were used as control groups. MMP fluorescent activity is shown in green. Acid-etched dentin treated with FS/FX-50, 100, and 200 resulted in weaker fluorescence, indicating reduced endogenous enzymatic activity. 50 µg/mL, 100 µg/mL, and 200 µg/mL FS and FX (FS-50, FS-100, FS-200, FX-50, FX-100, and FX-200 respectively).