Table 3.
Blood biochemical parameters of broiler chickens fed different levels of SBPM.
| Parameters | Thermoneutral (SBPM, ppm) | Heat-Stressed (SBPM, ppm) | SEM | Two-Way ANOVA | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 75 | 150 | 300 | 0 | 75 | 150 | 300 | Temp. | Diet | Temp. × Diet | ||
| TBARS | 38.6 c | 31.7 d | 31.1 d | 33.8 cd | 58.2 a | 50.7 b | 48.1 bc | 43.2 c | 2.7 | p < 0.001 | p < 0.001 | 0.045 |
| CORT | 24.3 c | 26.2 c | 25.1 c | 28.1 c | 53.8 a | 51.4 a | 48.3 ab | 40.6 b | 2.8 | p < 0.001 | 0.019 | 0.047 |
| UA | 77.6 c | 80.1 c | 82.2 bc | 76.4 c | 101.5 a | 86.7 b | 88.0 b | 85.8 b | 3.5 | p < 0.001 | 0.012 | 0.002 |
| IL-6 | 37.0 d | 35.7 d | 34.0 d | 36.7 d | 87.6 a | 73.4 b | 66.4 bc | 62.4 c | 3.0 | p < 0.001 | p < 0.001 | p < 0.001 |
| IL-1β | 9.5 | 8.2 | 8.0 | 8.3 | 29.7 | 25.9 | 22.0 | 20.7 | 2.5 | p < 0.001 | 0.024 | 0.135 |
| FITC-d | 0.28 c | 0.32 c | 0.32 c | 0.31 c | 0.64 a | 0.48 b | 0.52 b | 0.50 b | 0.03 | p < 0.001 | 0.023 | p < 0.001 |
Data are means of twelve birds. Data were analyzed using two-way ANOVA with Tukey’s multiple-comparison test. Different superscript letters indicate statistically significant differences (abcd p < 0.05). A unit of each parameter is as follows: TBARS, nmol/mL; FITC-d, ug/mL; CORT, ng/mL; UA, nmol/mL; IL-6, pg/mL; IL-1β, pg/mL. Abbreviations: TBARS, 2-thiobarbituric acid reactive substance; FITC-d, fluorescein isothiocyanate-dextran; CORT, corticosterone; UA, uric acid; IL, interleukin.