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. 2024 Nov 29;13(23):1978. doi: 10.3390/cells13231978

Figure 1.

Figure 1

Figure 1

Analyses of RNA-seq data from sc-islets showed enrichment of autoimmune disease genes. (A) Images of unfixed HSV-1-infected sc-islets, HSV-1-infected and ACV-treated sc-islets, and uninfected sc-islets after 48 h. (B) PCA plot of the sc-islet RNA-seq data showing clustering of uninfected sc-islets (in blue), infected sc-islets (in red), and HSV-1-infected and ACV-treated sc-islets (in orange) along PC1 (x-axis). (C) Plot showing GSEA results from comparisons of the sc-islet RNA-seq datasets across 21 common diseases. The labels are “NDD” for neurodegenerative diseases, “NPD” for neuropsychiatric disorders, “AID” for autoimmune diseases, and “Traits” for traits and related diseases. The y-axis shows the -log10(nominal p-value from the GSEA result) and the x-axis shows each disease: AD for Alzheimer’s disease, ALS for amyotrophic lateral sclerosis, MS for multiple sclerosis, PD for Parkinson’s disease, ADHD for attention deficit hyperactivity disorder, ASD for autism spectrum disorder, BD for bipolar disorder, MDD for major depressive disorder, OCD for obsessive-compulsive disorder, SCZ for schizophrenia, TS for Tourette’s syndrome, CD for Crohn’s disease, IBD for inflammatory bowel disease, PSO for psoriasis, RA for rheumatoid arthritis, T1D for type 1 diabetes, T2D for type 2 diabetes, BMI for body mass index, FBRAIN for FMRI brain measurements, HEIGHT for height, WAISTHIP for waist-to-hip ratio. The comparison groups are: HSV-1-infected and ACV-treated versus uninfected sc-islets (ACV versus Ctrl, in red), HSV-1-infected versus HSV-1-infected and ACV-treated sc-islets (Inf versus ACV), and HSV-1-infected versus uninfected sc-islets (Inf versus Ctrl, in blue). (D) Heatmap showing the normalized log2 (FPKM+1) expression for the top 50 DEGs sorted by the adjusted p-values for uninfected sc-islets, infected sc-islets, and ACV-treated sc-islets. (E) Plot to show GSEA results from previously published RNA-seq datasets from HSV-1-infected 3D cerebral organoids. For the GSE145496 dataset, we used the 8dpi samples (mock versus HSV-1-infected) for the analyses.