Figure 5.

Chromosome aberrations are contingent with the lack of PER expression in glia. (A) The overexpression of PER (driving transcription of UAS-per) only in the glia of per⁰ larvae [repo-GAL4 > PER (per⁰)] drastically reduced the frequency of aberrations [(abnormal metaphases/total metaphases) × 100]. Chi-square = 42.21, df = 2, **** p < 0.0001. Total number of metaphases scored (from left to right), n = 612, 302, 1021. ZT1. (B) The knock-out of per (with CRISPR/Cas9) only in glia (repo-GAL4 > gRNAper, CAS9) was sufficient to trigger chromosome aberrations. repo-GAL4 > gRNAacp, CAS9 vs. repo-GAL4 > gRNAper, CAS9, Fisher’s exact test, * p = 0.0161. Total number of metaphases scored, n = 386, 164. ZT = 1.