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Dose-dependent IL-8 secretion induced by HeLa cell and skeletal muscle CF in WT and CD36-overexpressing HEK293 cells. WT and CD36-overexpressing HEK293 cells were incubated with increasing concentrations of the CF preparations (see Section 2) from HeLa cells, c-CF (A) or murine skeletal muscle, SM-CF (B) for 20 h. IL-8 levels were quantified in duplicate samples of cell culture supernatants by ELISA. Data represent one of three separate experiments that yielded similar results.
