Figure 2.

Dose-dependent IL-8 secretion induced by the HMGB1 and LPS in WT and CD36-overexpressing HEK293 cells. Effect of heat treatment on the cytokine-inducing activity of HMGB1 and LPS. WT and CD36-overexpressing HEK293 cells were incubated with increasing concentrations of recombinant HMGB1 (A) or LPS (B) for 20 h. IL-8 levels were quantified in duplicate samples of cell culture supernatants by ELISA. Data represent one of three separate experiments that yielded similar results. The IL-8 levels were determined using duplicate samples of cell culture supernatants collected after a 20 h incubation of cells with either intact or heat-treated (100 °C for 45 min) HMGB1 (C) and LPS (D). The data presented are from one of two separate representative experiments.