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. 2024 Dec 3;13(23):1992. doi: 10.3390/cells13231992

Figure 4.

Figure 4

Dose-dependent IL-8 secretion induced by the histone H3 in WT and CD36-overexpressing HEK293 cells. Effect of L-37pA on hH3-induced IL-8 secretion in CD36-HEK293 cells. WT and CD36-overexpressing HEK293 cells were incubated with increasing concentrations of histone H3 for 20 h. IL-8 levels were quantified in duplicate samples of cell culture supernatants by ELISA (A). Data represent one of three separate experiments that yielded similar results. CD36-HEK293 cells were preincubated for 1 h with 0, 10 µg/mL, and 25 µg/mL of L-37pA or L3D peptides before a 20 h treatment with 25 µg/mL of histone H3. IL-8 levels were determined in cell culture supernatants in duplicate (B). * p < 0.05, ** p < 0.01, and ns—nonsignificant, versus histone H3 alone. Data are from one of at least two representative experiments.