Table 1.
Details for the eight forward and seven reverse RPA oligos designed for the amplification of region 1 and/or region 2 on the maxicircle kinetoplast.
| Primer Name | Nucleotide Sequence | Length | Nt. Location in PSC1 Kinetoplast ACC#BK010875 |
|---|---|---|---|
| F1 | GGCAAGTCCTACTCTCCTTTACAAAG | 26 | 1808..1838 |
| F2 | GGCAAGTCCTACTCTCCTTTACAAAGAGAAC | 31 | 1808..1833 |
| F3 | TTGTATGTTTGATTGGGGCAATACT | 25 | 1851..1875 |
| F4 | AGGTTCGAGCAGGTTAACAAGC | 22 | 1922..1943 |
| F5 | ATGTGTTTCATCGTCTACTTATTGC | 25 | 1955..1979 |
| F6 | TTCGTTAGTTGGGTTAAAATCGTTG | 25 | 2012..2036 |
| F7 | GATGCCAGCCGTTGCGGTAATTTCTATGC | 29 | 2417..2445 |
| F8 | GATGCCAGCCGTTGCGGTAATTTC | 24 | 2417..2440 |
| R1 | ATTAATGCTTGTTAACCTGCTCGAAC | 26 | rev:1924..1949 |
| R2 | TAGCAATAAGTAGACGATGAAACAC | 25 | rev:1957..1981 |
| R3 | TGCTTTACAACGATTTTAACCCAAC | 25 | rev:2019..2043 |
| R4 | TGCTTTACAACGATTTTAACCCAACTAACG | 30 | rev:2014..2043 |
| R5 | TAAAAGCATAGAAATTACCGCAACG | 25 | rev:2426..2450 |
| R6 | GTTGTCTTTATTACAAAGAATGGTGGGCAAC | 31 | rev:2738..2768 |
| R7 | GTTGTCTTTATTACAAAGAATGGTG | 25 | rev:2744..2768 |