Table 2.
Details for the six CRISPR guides designed for the detection of all Leishmania and mucocutaneous specific species. PAM sequence in bold is not included in the oligo.
| Guide Name | Nucleotide Target Sequence | Length | Location on Maxicircle |
|---|---|---|---|
| All-1 | TTTACAACGATTTTAACCCAACTAA | 25 (21 + PAM) | rev:2016..2040 |
| All-2 | TTTAGGAATAGTTAATAATAATTTA | 25 (21 + PAM) | 2284..2308 |
| All-3 | TTTGACAACATGATAAGGATTATAA | 25 (21 + PAM) | 2629..2653 |
| All-4 | TTTATAAAATAAATGTATAATATTT | 25 (21 + PAM) | rev:2450..2474 |
| MC-1 | TTTAAAAATATAAAAGTCAATTGTT | 25 (21 + PAM) | 2138..2161 |
| MC-2 | TTTATATTATTTTATATTATTTTAT | 25 (21 + PAM) | 2178..2201 |