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Experimental protocol. Supernatants harvested from BLM-loaded FG or non-loaded fibrin glue coated in media at varying time intervals were transferred to spheroid cultures in multi-well plates. Five experimental arms: non-treatment controls, (2) RT only, (3) BLMFG +RT, (4) BLMFG (supernatant harvested from BLM-loaded FG), (5) BLMFG +PCI + RT.
