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The neurovascular imaging of in vivo adult intact mouse brain at 1650 nm excitation with 2-photon fluorescence for Q820@BSA labeled blood vessels and 3-photon fluorescence for RFP labeled the neurons. At a depth of 1000 µm, white matter is observed, and we can go beyond the dense neurons in the hippocampus layer observed at 1200 µm. Scalar bar: 100 µm.
