Skip to main content
. 2024 Nov 26;13(23):1960. doi: 10.3390/cells13231960

Figure 3.

Figure 3

Indirect immunofluorescence of biomarkers under study in GK, EPI and FIB phenotypes of 4-day-old cultures, within the subconfluent culture stage. (A–C) Vimentin (VIM) expression in all phenotypes with decreasing intensity and changed cellular topology in FIBs (A), EPIs (B) and GKs (C). (D–F) Cytokeratin 5 staining (KRT5) in FIBs, EPIs and GKs with increasing intensity and different cellular topology. (G) Involucrin (IVL) staining in FIBs, (H) EPIs and (I) GKs with changed cytoplasmic distribution. (J–L) Loricrin (LOR) staining in FIBs was almost not detectable (J) and in EPIs (K) and GKs (L) with a faint cytoplasmic per-nuclear distribution. Cytokeratin 10 (KRT10) staining in FIBs (M) was again almost not detectable and in EPIs (N) and GKs (O) detectable with an inhomogeneous cytoplasmic distribution. (P–R) Lamin B1 (LMNB1) staining in FIBs (P), EPIs (Q) and GKs (P) with nuclear localization. Biomarker under study with green fluorescence, red cytoskeleton staining in red and DAPI nuclear counterstain in blue. 60× magnification. Bars correspond to 100 µm.