Table 2.
Primer sequences of working STS markers derived from RFLP marker psr1205 and SLAF sequences 264410, 4607154, and 6805321.
| Marker | Chromosome Location | Primer Name | Primer Sequence 5′ --> 3′ | TA °C | Amplicon bp |
|---|---|---|---|---|---|
| Psr1205 | chr3B:541288907..541289382 | Forward | CGGCAATGATGAGTGTGTCAT | 59 to 56 | 362 |
| Reverse | CAACTCCCAGTTTGCTGACA | ||||
| Marker264410 | chr3B:558511365..558511884 | Forward | ACACTACTCATACGGAACCATCG | 55 | 462 |
| Reverse | TCTTGGCTGACTTGGCATTCA | ||||
| Marker4607154 | chr3B:558833142..558833625 | Forward | ACAAGCAACTAACAGAGCCA | 55 | 486 |
| Reverse | CTGTCGATGCAGGGTTCTACT | ||||
| Marker6805321 | chr2E:49152430..49152911 | Forward | AATGTGAACAATCAACGAGATGT | 52 | 377 |
| Reverse | GTGCACAACACACAGTGGTC |