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Effect of 1,4-dioxane on the association constants between DPH and HSA. (A,B) Titration of DPH with HSA in water. (C,D) Titration of DPH with HSA in 1,4-dioxane (10%). (E,F) Titration of DPH with HSA in 1,4-dioxane (20%). The association constants were obtained by fitting the data in Equation (2).
