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The relevance of bulk solvent dielectric constant on binding constants between DPH and HSA. The dielectric constant of water was modified by adding 1,4-dioxane. The results are the means and SD of triplicates. Ka (20%) 20.6 ± 5.2 × 105 M−1, (10%) 4.5 ± 0.7 × 105 M−1, and (0%) 1.7 ± 0.3 × 105 M−1.
