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. 2024 Nov 30;25(23):12901. doi: 10.3390/ijms252312901

Figure 5.

Figure 5

CstC mediates resistance to cystocin. (A) Structural alignment of the CstC AlfaFold 3 model (grey) with the PAC-PuroOAc-CoA complex (pink), PDB ID 7K0A. (B) Antibiotic-binding pocket of CstC and PAC. The 31 and 49 positions located proximately to the aminoacyl residue of Puro-OAc are highlighted with sticks. (C) Comparison of bacterial susceptibility for E. coli ΔtolC and E. coli ΔtolC producing CstC or PAC. (D) Cytotoxicity of Cst and Puro toward human cell line HEK293T and HEK293T producing CstC or PAC. (E) Heat map of MICs of Cst and Puro against Gram-positive and Gram-negative bacteria.