Figure 6.

Cst mode of action. (A) Schematic representation of Puro mode of action. In vitro prokaryotic (B) and eukaryotic (C) protein synthesis inhibition by Puro and Cst. (D) Fluorescently labeled short peptides synthesis inhibition assay scheme, labeled peptides MF₂ and MF₄ are synthesized with coupled transcription–translation from exogenically formed loaded tRNA and visualized using PAGE with fluorescent detection. BPY—BODIPY. (E) Heatmap of normalized intensity of full-length peptides under treatment with Cst/Puro. (F) The principal scheme of toe-printing analysis. (G) Toe-printing analysis for Cst/Puro. The green arrow indicates the position of ribosome capture on the Thr codon; the blue arrow indicates the start codon. Ths—thiostrepton.