Table 2.

Sequences of the forward (F) and reverse (R) primers used for the amplification of the hypervariable regions of the 16S rRNA gene, as well as Denaturation (D), Annealing (A), and Extension (E) temperatures induced during PCR amplification with each primer pair.

16S Gene Region	Primer Pair	PCR Conditions
V1–V3 (27F-533R)	F: AGA GTT TGA TCM TGG CTC AG R: TTA CCG CGG CKG CTG GCA CG	D: 95 °C 1 min A: 30 × [95 °C 20 s, 56 °C 30 s, 65° 1 min] E: 65 °C 5 min
V3–V4 (341F-805R)	F: CCT ACG GGN GGC WGC AG R: GAC TAC HVG GGT ATC TAA TCC	D: 95 °C 1 min A: 30 × [95 °C 20 s, 56 °C 30 s, 65° 1 min] E: 65 °C 5 min
V1–V9 (27F-1492R)	F: AGA GTT TGA TCM TGG CTC AG R: CGG TTA CCT TGT TAC GAC TT	D: 95 °C 1 min A: 30 × [95 °C 20 s, 52 °C 30 s, 65° 2 min] E: 65 °C 5 min