Effect of HCA preconditioning on cardiac I/R injury-induced expression of multiple cytokines in the three groups determined using cytokine array analysis. (A) Original cytokine expression. (B) Matching dot plot. The expression levels of TCK-1 (C), IL-1 R6 (D), ICAM-1 (E), MMP-8 (F), agrin (G), TNF-α (H), IL-2 (I), IL-4 (J), IL-10 (K), LIX (L), CINC-1 (M), and prolactin (N) are significantly elevated in I/R vs. sham group, whereas they are significantly decreased in HCA+I/R vs. I/R group. All data are expressed as mean ± SEM (n = 4). * indicates significant differences vs. sham group (** p < 0.01; *** p < 0.001; **** p < 0.0001). # indicates the significant differences vs. I/R group (# p < 0.05; ## p < 0.01; ### p < 0.001; #### p < 0.0001).