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. 2024 Nov 27;25(23):12748. doi: 10.3390/ijms252312748

Figure 2.

Figure 2

Analysis of expression level of StPYL20 gene. (a) Tissue-specific expression of StPYL20. Values denoted by different lowercase letters within distinct organs are significantly different (p < 0.05). (b,c) The expression levels of StPYL20 under drought (b) and ABA (c) stress. Three-week-old potato seedlings were subjected to stress treatment in 1/2MS liquid medium containing a final concentration of 200 mM mannitol and 100 µM ABA. Whole plants were collected for RNA extraction after 0, 1, 3, 6 and 12 h of stress, and the expression level of StPYL20 was detected by qRT-PCR. The 2−ΔΔCT method was used to evaluate the relative expression, and the expression levels of genes in root tissue and stress for 0 h were defined as “1”. Mean values from three independent biological replicates, each with 9 plants from each line, +/− SD, are shown. Statistical significance of differences between groups was determined by one-way ANOVA (with Tukey’s test). ** indicates significant difference at p < 0.01 level compared to the control group (0 h).