Determination of stress-related physiological index contents in different plants under different treatment conditions. The physiological indicators mainly include Pro content, MDA content, and antioxidant enzyme activity. Mean values from three independent biological replicates, each with 9 plants from each line, +/− SD, are shown. Statistical significance of differences between groups was determined by one-way ANOVA (with Tukey’s test). *, ** denote statistical significance at p < 0.05 and p < 0.01 levels, respectively. ‘ns’ signifies no significant difference. A horizontal line above columns indicates data points with similar levels of significance compared to the wild-type control.