Figure 7.

Analysis of stress-responsive genes in transgenic and WT plants under normal and drought stress. The total RNA was isolated from transgenic and wild-type tobacco after 30 days under 100 mM mannitol treatments. The 2^−ΔΔCT method was used to evaluate the relative expression, and the expression levels of genes in the WT plants under normal conditions were defined as “1”. Mean values from three independent biological replicates, each with 9 plants from each line, +/− SD, are shown. Statistical significance of differences between groups was determined by one-way ANOVA (with Tukey’s test). **, denote statistically significant differences at p < 0.05 levels.