Figure 5.

CalPegA depletes plasma asparagine in vivo but does not affect eIF4E and 4EBP1 phosphorylation status. (A) Plasma was isolated from whole blood of AML45-luc tumor-bearing mice and concentrations of glutamine and asparagine (µM) were measured. Statistical analyses to compare vehicle and treatment groups were performed using unpaired t-tests. **** p < 0.0001, * p < 0.05. (B) Western blot analysis of bone marrow cells isolated from AML45-luc tumor-bearing mice. Lysates were probed with the indicated antibodies, and results are expressed as the ratio of phosphorylated to total eIF4E and 4EBP1, normalized to loading control (actin). Unpaired t-tests were performed to compare vehicle to treatment groups and no statistically significant differences were observed.