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Primers used in PCR analysis.
| Genes | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|
| gh | TGCTATTGTCGGTGGT | CTGTCTGCGTTCCTCA |
| npy | TGCTTGGGAACTCTAACGGAA | GACCTTTTGCCATACCTCTGC |
| agrp1 | CCGTGCATCCCTCATCAGC | GCTACGGCAGTAGCAGAAGGC |
| orxin | AATCCTGACGATGGGAAAGAG | TCGTGGTTTTAGCGACAAGTG |
| β-actin | GATGATGAAATTGCCGCACTG | ACCAACCATGACACCCTGATGT |
| cyp17a1 | GTGGCATTGAAGAATTCGCAT | CCGTATTTCTTCTGCAGCTGC |
| Sex marker | GAGCATCCACTGTCAACTT | ACTCTTCCCAAACACTGATT |
