Figure 1.

Automatic segmentation of TMA cores by MALDI-MSI. (A) Comparison of MALDI-MSI segmentation images with the corresponding H&E image of a representative cohort of TMA cores. (B) Distinction of NIFTPs (red- and yellow-colored regions) from the surrounding thyroid parenchyma (green-colored areas) and fibrotic areas (purple) through bisecting K-means clustering of the TMA cores based on the proteomic MSI data. H&E, hematoxylin and eosin; MALDI-MSI, matrix-assisted laser desorption/ionization mass spectrometry imaging; NIFTP, noninvasive follicular thyroid neoplasm with papillary-like nuclear features; TMA, tissue microarray.