Figure 2.

Biological properties, lytic activity, and stability of JSSK01. (A) Approximately 95% of the phage was adsorbed onto the host multidrug-resistant (MDR) E. coli 70751 within 6 min and was completely adsorbed by 10 min. (B) One-step growth curve of JSSK01 on MDR 70751. The phage undergoes a 25-min latent period, releasing approximately 123 phage-forming units (PFUs) per infected cell at 35 min. (C) JSSK01 lytic activity on MDR 70751. A 0.5 OD₆₀₀ of MDR 70751 was used to infect the phage at different MOIs and monitored for 12 h by measuring the OD₆₀₀ every 30 min. (D) JSSK01 stability was assessed by exposing it to different temperatures, ranging from 25 °C to 65 °C, for 1 h. ** indicates p < 0.01; NS denotes no significance. (E) JSSK01 stability was assessed by incubating 10⁸ PFU/mL phage titers in pH-adjusted LB media for 1 h. Plaque assays were performed to determine phage survival after treatment. All the experiments were performed in triplicate; the error bars represent the standard error of the mean.