Figure 5.

JSSK01 can rescue MDR 70751-infected zebrafish. (A) A lethal dose assessment was conducted on three groups of adult zebrafish, each comprising 10 fish. These groups were subjected to intraperitoneal injections of MDR 70751 at various concentrations (10⁸, 10⁷, and 10⁶ CFU in 20 µL of media, as well as 0.85% NaCl as the negative control, represented by the black, red, green, and purple lines, respectively). (B) Zebrafish were subjected to JSSK01 treatment (MOI = 10, 1, 0.1) for 30 min after bacterial infection with 10⁷ CFU (black line, red line, green line). Additionally, a group received JSSK01 treatment alone (2.5 × 10⁷ CFU/20 µL, purple line), whereas a negative control group received 0.85% NaCl (orange line). (C) The manifestation of disease symptoms in zebrafish infected with E. coli 70751 alone was compared with that in the groups rescued by the phage (MOI = 10) and the control group (0.85% NaCl). The views provided are as follows: left, side view; middle, top view; and right, abdominal anatomy. Kaplan–Meier Survival curves were plotted, with the X-axis denoting days post-infection and the Y-axis indicating the survival percentage. Statistical analysis was conducted via log-rank and generalized Wilcoxon tests in GraphPad Prism 9 software, where *** and * indicate p < 0.001 and p < 0.05, respectively.