Abstract
The transport and oxidation of succinate by functionally intact Ehrlich ascites-tumour cells was investigated. On the basis of pH dependence and inhibitor sensitivity it was concluded that succinate may be transported across the cell membrane by the organic anion carrier system. Thus the ability of isolated Ehrlich cells to oxidize succinate is real, and is not necessarily a result of damage to cell integrity.
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- Archakov A. I., Karyakin A. V., Skulachev V. P. Intermembrane electron transfer in mitochondrial and microsomal systems. FEBS Lett. 1974 Feb 15;39(2):239–242. doi: 10.1016/0014-5793(74)80059-1. [DOI] [PubMed] [Google Scholar]
- Aubert L., Motais R. Molecular features of organic anion permeablity in ox red blood cell. J Physiol. 1975 Mar;246(1):159–179. doi: 10.1113/jphysiol.1975.sp010884. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Babcock D. F., First N. L., Lardy H. A. Transport mechanism for succinate and phosphate localized in the plasma membrane of bovine spermatozoa. J Biol Chem. 1975 Aug 25;250(16):6488–6495. [PubMed] [Google Scholar]
- Bhargava P. M., Siddiqui M. A., Kumar G. K., Prasad K. S. Effect of cell concentration on the uptake of amino acids by rat liver parenchymal cells in suspension. J Membr Biol. 1975 Jul 24;22(3-4):357–368. doi: 10.1007/BF01868180. [DOI] [PubMed] [Google Scholar]
- DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
- Dubinsky W. P., Cockrell R. S. Ca2+ transport across plasma and mitochondrial membranes of isolated hepatocytes. FEBS Lett. 1975 Nov 1;59(1):39–43. doi: 10.1016/0014-5793(75)80336-x. [DOI] [PubMed] [Google Scholar]
- Dubinsky W. P., Cockrell R. S. Respiratory control and mitochondrial monovalent cation permeability of isolated liver cells. Biochem Biophys Res Commun. 1974 Jan 23;56(2):415–422. doi: 10.1016/0006-291x(74)90858-4. [DOI] [PubMed] [Google Scholar]
- Hems R., Stubbs M., Krebs H. A. Restricted permeability of rat liver for glutamate and succinate. Biochem J. 1968 May;107(6):807–815. doi: 10.1042/bj1070807. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kleineke J., Stratman F. W. Calcium transport in isolated rat hepatocytes. FEBS Lett. 1974 Jul 1;43(1):75–80. doi: 10.1016/0014-5793(74)81109-9. [DOI] [PubMed] [Google Scholar]
- Landry Y., Lehninger A. L. Transport of calcium ions by Ehrlich ascites-tumour cells. Biochem J. 1976 Aug 15;158(2):427–438. doi: 10.1042/bj1580427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mapes J. P., Harris R. A. On the oxidation of succinate by parenchymal cells isolated from rat liver. FEBS Lett. 1975 Mar 1;51(1):80–83. doi: 10.1016/0014-5793(75)80858-1. [DOI] [PubMed] [Google Scholar]
- Penttila A., Trump B. F. Studies on the modification of the cellular response to injury. II. Electron microscopic studies on the protective effect of acidosis on anoxic injury of Ehrlich ascites tumor cells. Virchows Arch B Cell Pathol. 1975;18(1):1–16. doi: 10.1007/BF02889230. [DOI] [PubMed] [Google Scholar]
- Ross B. D., Hems R., Krebs H. A. The rate of gluconeogenesis from various precursors in the perfused rat liver. Biochem J. 1967 Mar;102(3):942–951. doi: 10.1042/bj1020942. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spencer T. L., Lehninger A. L. L-lactate transport in Ehrlich ascites-tumour cells. Biochem J. 1976 Feb 15;154(2):405–414. doi: 10.1042/bj1540405. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stubbs M., Krebs H. A. The accumulation of aspartate in the presence of ethanol in rat liver. Biochem J. 1975 Jul;150(1):41–45. doi: 10.1042/bj1500041. [DOI] [PMC free article] [PubMed] [Google Scholar]