| Component | Volume (μL) | Final concentration |
|---|---|---|
| 10× reaction buffer | 2 | 1× |
| ATP 100 mM | 2 | 10 mM |
| CTP 100 mM | 2 | 10 mM |
| TTP 100 mM | 2 | 10 mM |
| UTP 100 mM | 2 | 10 mM |
| T7 RNA pol Mix | 2 | — |
| DNA (500 ng) from Step 9 | 2 | 25 ng/μL |
| Nuclease-free water | 6 | — |
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