| Cycle no. | Denature | Anneal | Extend | Final |
|---|---|---|---|---|
| 1 | 98 °C, 2 min | |||
| 2–34 | 98 °C, 30 s | 58 °C, 30 s | 72 °C, 2 min 30 s | |
| 35 | 72 °C, 5 min | |||
| 36 | 16 °C, hold |
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