Table 2.
Statistically significant lipids in liver biopsies that contribute to the discrimination between the healthy controls and patients with NASH and NAFL–NASH patients. p values, adjusted p* values, Log2FC, CV%, and VIP scores are presented for the TG species. Bolded values correspond to p ≤ 0.05.
| Lipids | Lipid Species | Adjusted p* Values | Controls–NASH | NAFL–NASH | |||||
|---|---|---|---|---|---|---|---|---|---|
| p Value | VIP | Log2FC | p Value | VIP | Log2FC | CV% | |||
| TG 42:0 | TG 10:0_16:0_16:0 TG 12:0_14:0_16:0 TG 14:0_14:0_14:0 |
2.50 × 10−2 | 7.10 × 10−3 | 0.8 | 3.17 | 2.10 × 10−2 | 0.9 | 3.55 | 3.79 |
| TG 42:1 | TG 10:0_14:0_18:1 TG 10:0_16:0_16:1 TG 12:0_12:0_18:1 TG 12:0_14:0_16:1 TG 12:0_14:1_16:0 TG 14:0_14:0_14:1 TG 8:0_16:0_18:1 |
7.20 × 10−3 | 3.40 × 10−2 | 0.7 | 2.37 | 3.10 × 10−3 | 0.9 | 3.42 | 4.23 |
| TG 44:0 | TG 12:0_16:0_16:0 TG 14:0_14:0_16:0 |
5.80 × 10−3 | 3.30 × 10−3 | 1.1 | 3.04 | 4.30 × 10−2 | 1.3 | 3.1 | 3.55 |
| TG 44:1 | TG 10:0_16:0_18:1 TG 12:0_14:0_18:1 TG 12:0_16:0_16:1 TG 14:0_14:0_16:1 |
9.50 × 10−3 | 3.30 × 10−3 | 1.4 | 3.21 | 4.30 × 10−2 | 1.6 | 3.62 | 3.07 |
| TG 44:2 | TG 10:0_16:0_18:2 TG 10:0_16:1_18:1 TG 12:0_14:0_18:2 TG 12:0_14:1_18:1 TG 12:0_16:0_16:2 TG 12:0_16:1_16:1 TG 14:0_14:1_16:1 TG 14:1_14:1_16:0 TG 8:0_18:1_18:1 |
3.00 × 10−2 | 5.80 × 10−3 | 0.9 | 2.91 | 4.10 × 10−2 | 1.1 | 3.57 | 3.67 |
| TG 46:0 | TG 14:0_14:0_18:0 TG 14:0_16:0_16:0 |
3.30 × 10−2 | 3.30 × 10−3 | 1 | 1.96 | 4.30 × 10−2 | 1.2 | 2.03 | 1.68 |
| TG 46:1 | TG 12:0_16:0_18:1 TG 14:0_14:0_18:1 TG 14:0_16:0_16:1 |
3.30 × 10−5 | 2.30 × 10−3 | 2.3 | 3.32 | 3.80 × 10−2 | 2.6 | 2.84 | 2.42 |
| TG 46:2 | TG 10:0_18:1_18:1 TG 12:0_16:0_18:2 TG 12:0_16:1_18:1 TG 14:0_14:0_18:2 TG 14:0_14:1_18:1 TG 14:0_16:1_16:1 TG 14:1_16:0_16:1 |
7.10 × 10−3 | 2.80 × 10−3 | 1.8 | 3.28 | 3.20 × 10−2 | 2.1 | 3.41 | 3.12 |
| TG 46:3 | TG 10:0_18:1_18:2 TG 12:0_16:1_18:2 TG 14:0_14:1_18:2 TG 14:1_14:1_18:1 TG 14:1_16:1_16:1 |
1.40 × 10−2 | 3.40 × 10−3 | 1 | 3.18 | 2.60 × 10−2 | 1.1 | 3.47 | 3.79 |
| TG 48:0 | TG 14:0_16:0_18:0 | 2.80 × 10−2 | 2.30 × 10−3 | 1 | 1.86 | 3.80 × 10−2 | 1.2 | 1.69 | 1.77 |
| TG 48:1 | TG 14:0_16:0_18:1 TG 14:0_16:1_18:0 TG 15:0_15:0_18:1 TG 15:0_16:1_17:0 TG 16:0_16:0_16:1 |
2.30 × 10−5 | 9.90 × 10−4 | 2.5 | 2.54 | 7.50 × 10−2 | 2.6 | 1.6 | 1.8 |
| TG 48:2 | TG 12:0_18:1_18:1 TG 14:0_16:0_18:2 TG 14:0_16:1_18:1 TG 14:1_16:0_18:1 TG 16:0_16:0_16:2 TG 16:0_16:1_16:1 |
7.40 × 10−7 | 1.50 × 10−3 | 2.8 | 2.97 | 5.40 × 10−2 | 3 | 2.1 | 1.15 |
| TG 48:3 | TG 12:1_18:1_18:1 TG 14:0_16:1_18:2 TG 14:1_16:0_18:2 TG 14:1_16:1_18:1 TG 16:1_16:1_16:1 |
3.00 × 10−3 | 2.80 × 10−3 | 2.2 | 3.65 | 3.20 × 10−2 | 2.5 | 3.02 | 4.07 |
| TG 48:4 | TG 12:0_18:2_18:2 TG 14:1_16:1_18:2 |
9.90 × 10−3 | 5.00 × 10−3 | 1.1 | 3.89 | 1.80 × 10−2 | 1.2 | 3.08 | 3.35 |
| TG 49:0 | TG 15:0_16:0_18:0 TG 15:0_17:0_17:0 TG 16:0_16:0_17:0 |
3.50 × 10−2 | 1.90 × 10−3 | 0.7 | 2.17 | 4.50 × 10−2 | 0.8 | 1.97 | 1.88 |
| TG 49:1 | TG 14:0_17:0_18:1 TG 15:0_16:0_18:1 TG 15:0_17:0_17:1 TG 16:0_16:0_17:1 |
5.50 × 10−4 | 2.30 × 10−3 | 1.4 | 2.33 | 3.80 × 10−2 | 1.6 | 1.84 | 2.82 |
| TG 49:2 | TG 13:0_18:1_18:1 TG 14:0_17:0_18:2 TG 14:0_17:1_18:1 TG 15:0_16:0_18:2 TG 15:0_16:1_18:1 TG 15:0_17:1_17:1 TG 16:1_16:1_17:0 |
2.00 × 10−3 | 2.80 × 10−3 | 1.1 | 1.85 | 3.20 × 10−2 | 1.3 | 1.84 | 3.36 |
| TG 49:3 | TG 15:0_16:1_18:2 TG 15:1_16:0_18:2 TG 15:1_16:1_18:1 TG 15:1_17:1_17:1 TG 16:1_16:1_17:1 |
2.30 × 10−2 | 1.60 × 10−2 | 0.6 | 1.82 | 3.70 × 10−2 | 0.8 | 2.26 | 2.1 |
| TG 50:0 | TG 16:0_16:0_18:0 | 8.10 × 10−3 | 6.40 × 10−4 | 1.4 | 2.76 | 1.00 × 100 | 1.6 | 1.83 | 1.79 |
| TG 50:1 | TG 16:0_16:0_18:1 TG 16:0_16:1_18:0 |
6.50 × 10−5 | 4.10 × 10−4 | 3.3 | 2.29 | 1.40 × 10−1 | 3.4 | 1.37 | 1.11 |
| TG 50:2 | TG 14:0_18:0_18:2 TG 14:0_18:1_18:1 TG 16:0_16:0_18:2 TG 16:0_16:1_18:1 TG 16:0_16:2_18:0 TG 16:1_16:1_18:0 |
2.40 × 10−4 | 7.90 × 10−4 | 2.9 | 1.62 | 8.90 × 10−2 | 2.7 | 0.89 | 1.83 |
| TG 50:3 | TG 14:0_18:1_18:2 TG 16:0_16:0_18:3 TG 16:0_16:1_18:2 TG 16:0_16:2_18:1 TG 16:1_16:1_18:1 |
3.20 × 10−5 | 1.20 × 10−3 | 3 | 2.55 | 6.40 × 10−2 | 2.9 | 1.34 | 0.72 |
| TG 50:4 | TG 14:0_18:2_18:2 TG 14:1_16:1_20:2 TG 14:1_18:1_18:2 TG 16:1_16:1_18:2 |
3.10 × 10−3 | 2.30 × 10−3 | 2.4 | 3.92 | 3.80 × 10−2 | 2.6 | 2.67 | 3.92 |
| TG 50:5 | TG 14:0_14:0_22:5 TG 14:0_18:2_18:3 TG 14:1_18:1_18:3 TG 14:1_18:2_18:2 TG 14:2_18:1_18:2 TG 16:0_16:2_18:3 TG 16:1_16:1_18:3 TG 16:1_16:2_18:2 |
8.50 × 10−4 | 3.40 × 10−3 | 1 | 4 | 2.60 × 10−2 | 1.1 | 2.83 | 2.75 |
| TG 51:1 | TG 15:0_18:0_18:1 TG 16:0_16:0_19:1 TG 16:0_17:0_18:1 |
3.00 × 10−4 | 9.90 × 10−4 | 1.9 | 3.07 | 7.50 × 10−2 | 2.1 | 2.15 | 3.47 |
| TG 51:2 | TG 15:0_18:1_18:1 TG 16:0_17:1_18:1 |
8.80 × 10−6 | 1.20 × 10−3 | 0.9 | 2.28 | 6.40 × 10−2 | 0.9 | 1.3 | 4.61 |
| TG 51:3 | TG 16:0_17:1_18:2 TG 16:1_17:0_18:2 TG 16:1_17:1_18:1 TG 17:1_17:1_17:1 |
3.10 × 10−3 | 1.50 × 10−3 | 1.5 | 2.67 | 5.40 × 10−2 | 1.6 | 1.72 | 2.84 |
| TG 52:0 | TG 16:0_18:0_18:0 TG 16:0_16:0_20:0 |
1.70 × 10−3 | 2.30 × 10−3 | 1.1 | 2.57 | 3.80 × 10−2 | 1.2 | 2.14 | 3.81 |
| TG 52:1 | TG 16:0_18:0_18:1 | 1.80 × 10−4 | 9.90 × 10−4 | 3.8 | 2.97 | 7.50 × 10−2 | 3.9 | 1.56 | 1.53 |
| TG 52:2 | TG 16:0_18:0_18:2 TG 16:0_18:1_18:1 TG 16:1_18:0_18:1 |
3.10 × 10−3 | 1.20 × 10−3 | 3.7 | 1.2 | 1.00 × 100 | 3.1 | 0.49 | 1.32 |
| TG 52:3 | TG 16:0_16:0_20:3 TG 16:0_18:0_18:3 TG 16:0_18:1_18:2 TG 16:1_18:0_18:2 TG 16:1_18:1_18:1 |
1.80 × 10−2 | 2.10 × 10−3 | 2.9 | 0.97 | 1.50 × 10−1 | 2.4 | 0.4 | 2.5 |
| TG 52:4 | TG 16:0_16:1_20:3 TG 16:0_18:1_18:3 TG 16:0_18:2_18:2 TG 16:1_18:1_18:2 |
3.10 × 10−3 | 3.20 × 10−3 | 3.5 | 1.76 | 6.90 × 10−2 | 3.2 | 0.85 | 1.14 |
| TG 52:5 | TG 16:0_18:2_18:3 TG 16:1_18:1_18:3 TG 16:1_18:2_18:2 TG 16:2_18:1_18:2 |
1.20 × 10−2 | 2.70 × 10−3 | 2.7 | 3.52 | 5.10 × 10−2 | 2.9 | 2.11 | 3.75 |
| TG 53:1 | TG 16:0_17:0_20:1 TG 16:0_18:0_19:1 TG 16:0_18:1_19:0 TG 17:0_17:1_19:0 TG 17:0_18:0_18:1 TG 17:1_18:0_18:0 |
3.00 × 10−2 | 1.20 × 10−3 | 1 | 3.37 | 6.40 × 10−2 | 1.1 | 2.64 | 2.96 |
| TG 53:2 | TG 16:0_18:1_19:1 TG 17:0_17:1_19:1 TG 17:0_18:1_18:1 TG 17:1_18:0_18:1 |
1.30 × 10−3 | 9.90 × 10−4 | 1.9 | 2.73 | 7.50 × 10−2 | 1.9 | 1.47 | 3.56 |
| TG 53:3 | TG 16:0_18:2_19:1 TG 16:1_18:1_19:1 TG 17:0_18:1_18:2 TG 17:1_18:0_18:2 TG 17:1_18:1_18:1 |
8.30 × 10−3 | 2.60 × 10−3 | 1.5 | 2.28 | 8.10 × 10−2 | 1.4 | 1.08 | 2.74 |
| TG 54:1 | TG 16:0_16:0_22:1 TG 16:0_18:0_20:1 TG 18:0_18:0_18:1 |
3.30 × 10−2 | 1.20 × 10−3 | 2.2 | 4.01 | 6.40 × 10−2 | 2.4 | 3.08 | 4.71 |
| TG 54:2 | TG 16:0_18:0_20:2 TG 16:0_18:1_20:1 TG 18:0_18:0_18:2 TG 18:0_18:1_18:1 |
1.00 × 10−2 | 2.20 × 10−3 | 3.1 | 2.01 | 9.50 × 10−2 | 2.9 | 0.9 | 1.58 |
Adjusted p* Values: The value is adjusted with the values of the waist circumference and the HOMA-IR values.