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[Preprint]. 2024 Dec 5:2024.12.02.626426. [Version 1] doi: 10.1101/2024.12.02.626426

Figure 3. IFNγ promotes lymphatic differentiation away from an immature, tip-like state.

Figure 3.

A. Split UMAP scRNAseq analysis of tumor-associated LECs (LEC; Prox1+) sorted from B16-OVA-VEGFC tumors in Ifnγr1WT and Ifnγr1iProx1 mice from. B. Average expression of marker genes for each cluster, normalized from −1 to 1. Size indicates percentage of cells in the cluster expressing the transcript. C. Cluster proportion in the Ifnγr1WT and Ifnγr1iProx1 mice. D. Monocle 3 pseudotime analysis of the combined LEC object from (A). E-G. Gene expression as a function of pseudotime from (D). H. Heatmap of LEC fate genes expressed by human dermal LECs (HDLEC) treated with PBS or IFNγ (n=3, technical replicates) by bulk RNA sequencing.