Fig 6. Vaccination with INI-4001-adjuvanted POWV-I-VLP generates antibodies that bind POWV-II and LGTV and protects mice from lethal POWV-II challenge.

Mice prime-boost vaccinated 3–4 weeks apart sc with 10⁶ FFUe of VLP alone or adjuvanted with 300μg alum or 10nmol INI-4001. n=5/group. Mock group injected with PBS vehicle alone. (A) Serum collected 3 weeks post-boost and analyzed by whole-virus ELISA. Data are reported as log-transformed mean endpoint titer ± SEM. Statistical significance determined by one-way ANOVA with Šídák’s multiple comparisons test to compare vaccinated mice to mock-vaccinated mice. Data for POWV-II and LGTV represent one experiment; data for WNV represent independent experiment. n=5/group except for LGTV n=3 for mock and n=4 for INI-4001. (B-C) Mice challenged ip 3 weeks post-boost with 10⁵ FFU dose of POWV-II. Survival (B) and weights (C) monitored for 21 days post-challenge. (B) Statistical significance determined by Mantel-Cox log rank test to compare alum and INI-4001 groups. * = p = 0.05. (C) Weights represented as mean percent of initial weights ± SEM. (D) Area under the curve (AUC) of individual alum and INI-4001 survivors. Statistical significance determined by Student’s unpaired t-test. ** = p < 0.01. Data represent one experiment.