Extended Data Figure 5: Diffusion Plots of Tumor and Immune Cell Clusters Reveals Increased Activated CD8+ T-cell representation and Decreased Tumor Burden in BCR-rich samples.

A) Keratinocyte cells (identified in this study broadly as malignant cells) diffusion plot. DPT stands for diffusion pseudotime. The BCR clonotype count for each cell plotted is defined as the number of BCR clonotypes present in the tissue sample from which the plotted cell originated, representing the BCR diversity of its environment. Expression of EPCAM (BerEP4) a marker of basal cell carcinomas and progenitor keratinocytes, KRT1 an early differentiation marker, and IVL a late differentiation marker plotted per cell.

B) B and plasma cells aggregated diffusion plot. MHC-I expression is defined as the mean expression of all class I MHC genes per cell. IGH expression is defined as the mean expression of all heavy chain Ig genes per cell.

C) CD8+ T cells diffusion plot. Expression of CD69 and JUN, markers of TCR-induced activation plotted per cell.