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[Preprint]. 2024 Dec 6:2024.12.05.627054. [Version 1] doi: 10.1101/2024.12.05.627054

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Figure 2. — Sequence and oligomeric state of CrgA. (A) Sequence of the expressed CrgA, with TM α-helices in red and β-strands in blue. Ala55 is special in that its NH participates in TM1 hydrogen bonding but its CO participates in β3-β4 hydrogen bonding. Pro residues demarcating secondary structures are in bold. The nonnative His tag starts with LEH. (B) SDS-PAGE gels. Lanes: molecular weight ladder; 1–29 deletion mutant; G44V mutant; and the wild-type (WT) protein.