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. 2024 Nov 4;4(1):ycae134. doi: 10.1093/ismeco/ycae134

Figure 2.

Figure 2

Microbiome profiles of control and experimental flies. A–C. Scaled relative abundance plots for the 10 most abundant bacterial genera following antibacterial (AB), antifungal (AF) or antibacterial and antifungal (AB+AF) treatment and the respective controls (Control or COil). Each column represents the average of 6–8 individuals based on high throughput 16S rRNA gene amplicon sequencing. The p-values were determined using univariate multiple testing with an F test; *: Relative abundance is significantly different between control and treatment conditions. D–F. Scaled relative abundance plots for the 10 most abundant fungal genera following AB, AF, or AB+AF treatment or the respective controls (con or COil). Each column represents the average of 6–8 individuals based on high throughput ITS gene amplicon sequencing. The p-values were determined using univariate multiple testing with an F test; *: Relative abundance is significantly different between control and treatment conditions. G. Non-multidimensional scaling plots (NMDS) based on Jaccard distances of bacterial OTUs (grouped at 97% similarity level) from control and experimental treatments. Ellipses represent significance at 0.05 confidence. H. Non-multidimensional scaling plots (NMDS) based on Jaccard distances of fungal OTUs (grouped at 97% similarity level) from control and experimental treatments. Ellipses represent significance at 0.05 confidence.