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. 2024 Nov 28;29(23):5642. doi: 10.3390/molecules29235642

Figure 3.

Figure 3

Mass spectra of Fondaparinux (50 µM) and proportion of forms with at least one Na/K exchange on the MALDI-TOF spectra (i) after pretreatment on a monolithic cation-exchange column (L = 8.5 cm, di = 75 µm) functionalized with sodium bisulfite and on-line elution on the MALDI plate (gray trace); (ii) after pretreatment on a monolithic cation-exchange column (L = 8.5 cm, di = 75 µm) functionalized with sodium mercaptoethane sulfonate and on-line elution on the MALDI plate (gold trace); MALDI-TOF-MS detection between 1000 and 1800 Th. The samples are prepared with a HABA/TMG ½ 80 mg/mL matrix in methanol at a ratio of 0.5/1 µL of matrix/sample on a MALDI Anchorship plate. The spectra were obtained with a laser power of 73% for 100,000 spectra. Asterisks represent Na/K exchanges.