Figure 13. Computational modeling of a Kiss1^ARH neuron in the OVX and OVX + E2 state demonstrates its distinct dynamic responses.

A model of the Kiss1^ARH neuron was developed and calibrated using molecular data and electrophysiological recordings of Kiss1^ARH neurons from OVX and OVX + E2 mice. (A) Simulations of the OVX-parameterized model demonstrating high-frequency activity in response to saturating levels of NKB stimulation. The balance between GIRK and TRCP5 conductance controls the response of the neuron to NKB stimulation, with neuronal response eliminated when TRPC5 conductance is low (red triangle) relative to the GIRK conductance. (B) The OVX + E2 parameterized models demonstrate sustained burst firing activity. The bursting activity that is supported by elevated h- and Ca²⁺ currents (red square) as observed in OVX + E2 mice. (C) In the OVX + E2 state, burst firing activity is also supported by high conductance of HVA Ca²⁺ channels relative to the conductance of TRPC5 channels. Representative points in the parameter space giving rise to burst firing activity are marked with red squares, whereas red triangles are used for points resulting in regular spiking. The black line separates these two regions of activity.