Table 1. Primer table.
| Gene name (encodes for) | Accession number |
Primer location (bp) |
Product length (bp) |
Annealing temperature (°C) |
Efficiency slope |
r2 | % |
|---|---|---|---|---|---|---|---|
|
Cacna1c (Cav 1.2,L-type) |
NM_009781 | 1331–1348 1390–1407 |
77 | 60 | –3.478 | 0.989 | 94 |
|
Cacna1a (Cav 2.1,P/Q-type) |
NM_007578 | 6035–6054 6090–6109 |
75 | 60 | –3.498 | 0.980 | 93 |
|
Cacna1b (Cav 2.2,N-type) |
NM_001042528 | 5405–5426 5467–5488 |
84 | 60 | –3.483 | 0.992 | 94 |
|
Cacna1e (Cav 2.3,R-type) |
NM_009782 | 530–549 617–636 |
107 | 60 | –3.441 | 0.983 | 95 |
|
Cacna1g (Cav 3.1,T-type) |
NM_009783 | 5004–5025 5060–5083 |
80 | 60 | –3.372 | 0.968 | 98 |
| Hcn1 (HCN1) | NM_010408 | 1527–1546 1641–1662 |
136 | 60 | –3.253 | 0.958 | 100 |
| Hcn2 (HCN2) | NM_008226 | 1122–1143 1199–1218 |
97 | 60 | –3.279 | 0.969 | 100 |
| Kcnd2 (KCND2) | NM_019697 | 2135–2156 2217–2238 |
104 | 60 | –3.312 | 0.972 | 100 |
| Kcnn3 (SK3) | NM_080466 | 1259–1277 1352–1370 |
112 | 60 | –3.369 | 0.952 | 98 |
| Kcnma1 (BKα1) | NM_001253358 | 2745–2762 2833–2852 |
108 | 60 | –3.338 | 0.971 | 99 |
| Kcnb1 (KCNB1) | NM_008420 | 691–710 759–780 |
119 | 60 | –3.375 | 0.983 | 98 |
| Kcnq2 (KCNQ2) | NM_010611 | 1079–1098 1151–1170 |
92 | 60 | –3.367 | 0.978 | 98 |
| Tac2 (NKB) | NM_009312 | 368–389 490–511 |
144 | 60 | –3.440 | 0.989 | 95 |
| Slc17a6 (VGLUT2) | NM_080853 | 1275–1296 1371–1390 |
116 | 60 | –3.374 | 0.997 | 98 |
| Trpc5 (TRPC5)* | NM_009428 | 734–753 832–851 |
118 | 60 | –3.161 | 0.952 | 100 |
| Trpc5 (TRPC5)† | NM_009428 | 616–637 772–792 |
177 | 60 | –3.407 | 0.987 | 97 |
| Trpc5 (TRPC5)† | NM_009428 | 2083–2100 2162–2179 |
97 | 60 | –3.255 | 0.963 | 100 |
| Kcnj6 (GIRK2) | NM_001025584 | 488–507 592–609 |
122 | 60 | –3.368 | 0.919 | 98 |
| Gapdh (GAPDH) | NM_008084 | 689–706 764–781 |
93 | 60 | –3.352 | 0.998 | 99 |
*
Trpc5 primers (Figure 10E).
†
Trpc5 primers flanking sgRNA and PAM sites in mutagenesis (Figure 11D).