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. 2024 Dec 6;29(23):5782. doi: 10.3390/molecules29235782

Figure 2.

Figure 2

(a) Simulation of the HCR primers and products by NUPACK: the different colors of dots represented the equilibrium probability of hairpins primers and HCR products structures, the red box represented the concentrations of different hairpins primers and HCR products, the blue arrows represented the corresponding structure of HCR products; (b) agarose gel electrophoresis of HCR reaction: lane 1: miR-let7a (10 pmol) + HCR-MAB-let7a (10 pmol) + HCR-HP1 and HP2 (20 pmol), lane 2: HCR-MAB-let7a (10 pmol) + HCR-HP1 and HP2 (20 pmol), lane 3: HCR-HP1 and HP2 (20 pmol), lane 4: HCR-HP1 (20 pmol), lane 5: HCR-HP2 (20 pmol); (c) fluorescence spectrum of EvaGreen within the solution of HCR reaction products: 1: miR-let7a (2 pmol) + HCR-MAB-let7a (10 pmol) + HCR-HP1 and HP2 (20 pmol), 2: HCR-MAB-let7a (10 pmol) + HCR-HP1 and HP2 (20 pmol), 3: HCR-HP1 and HP2 (20 pmol), 4: HCR-HP1 (20 pmol), 5: HCR-HP2 (20 pmol).