Figure 1.

Experimental design and AgNP internalization quantification. (A) Flies were kept in 50 mL vials containing a small piece of paper and closed with a sponge plug. Flies were fed via a wetting sponge plug with 1 mL of peach–grape juice. Non-treated flies received only juice, while treated flies were exposed to AgNPs (100 and 400 µg/mL). (B) A photograph of a treated adult (as confirmed by the crop color). (C) Feces of the treated adults on the piece of paper. (D) ICP-MS analysis of silver in sponge plug wetted with juice (non-mixed or mixed with AgNPs), as well as in treated adult tissues and feces. Multiple pairwise comparisons (Turkey’s test) were performed using one-way ANOVA analysis. *** p < 0.001 and **** p < 0.0001.