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. 2024 Dec 6;14(23):1962. doi: 10.3390/nano14231962

Figure 3.

Figure 3

Cytotoxicity of MPEG-2000-DSPE-edaravone (MDE). (A) PC12 cells were treated with gradient concentrations of MDE (0, 3.125, 12.5, 50, and 200 μg/mL) for 72 h, and then cell viability was determined using CCK-8. For (B,C), a cell model of Parkinson’s disease was established in PC12 cells through exposure to rotenone (1 μM) for 24 h, which were then treated with MDE (12.5 and 50 μg/mL) or edaravone (2.1 and 8.4 μg/mL) for another 48 h. (B) Cell viability was determined using CCK-8. (C) Cells were stained with calcein acetoxymethyl ester (AM) and propidine iodide (PI) to evaluate cell living. Each experiment was repeated at least six times. Scale bars are 50 μm. * p < 0.05 versus blank group. ** p < 0.01 versus blank group.