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. 2024 Dec 4;16(23):4189. doi: 10.3390/nu16234189

Figure 6.

Figure 6

Evaluation of proteins regulating hepatocyte degeneration and liver fibrosis was conducted in HEPA-RG cell lines treated with sEVs isolated from patients with LR and patients with IHR, both before (T0) and after (T1) 8 weeks of VLCKD. Representative Western blots of various proteins (PIK3CB, AKT, pAKT, HDAC3, HDAC6, α-SMA, and VIM) and the housekeeping protein GAPDH (A). A semi-quantitative evaluation of protein expression levels was performed using video-densitometry analysis of PIK3CB, AKT, and pAKT1 (B), HDAC6 and HDAC3 (C), MMP2 and MMP9 (D), and α-SMA and VIM (E) bands on the Western blots. The GAPDH protein band was used to normalize the protein bands for each subject. (* p < 0.05 and ** p < 0.001 for T1 vs. T0).