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. 2024 Nov 22;13(23):3275. doi: 10.3390/plants13233275

Figure 4.

Figure 4

OsPik-2-like gene-knockout rice lines and their growth phenotypes. (a) Sanger sequencing analysis of mutation patterns in the target site of OsPik-2-like gene in the wild-type (WT) and OsPik-2-like knockout rice lines. Blue boxes represent exons. The sgRNA sequence that specifically targets OsPik-2-like is indicated. The mutations, an ‘‘A’’ insertion in line kopik2l-1 and a 16 bp deletion in kopik2l-16, lead to premature translational termination of OsPik-2-like. (b–i) Mean shoot height (b), root length (c), chlorophyll content (d), stem strength (e), shoot fresh weight (f), root fresh weight (g), shoot dry weight (h), and root dry weight (i) (+SE, n = 20) of WT plants and ko-pik2l lines at 30 days old in the phytotron. Asterisks indicate significant differences in ko-pik2l lines compared with WT plants evaluated by Bayesian analysis of variance (* p < 0.05 and *** p < 0.001). (j) The phenotype of 30-day-old WT and ko-pik2l lines in the phytotron.