Flavonoid levels and TrypPI activity in the leaves of WT plants and ko-pik2l lines. Mean concentrations (+SE, n = 5~6) of prunin (a), carlinoside (b), isovitexin (c), and mean TrypPI activity (+SE, n = 7) (d) in WT plants and ko-pik2l lines that were individually infested by a 3rd-instar LF larva on the first fully expanded leaf for 0 h or 48 h. Asterisks indicate significant differences in ko-pik2l lines compared with WT plants evaluated by Bayesian analysis of variance (* p < 0.05, ** p < 0.01 and *** p < 0.001). (e) Representative pictures of TrypPI after different treatments measured by radial diffusion assay.