Figure 5.

Effects of ABA on seed germination, cotyledon greening, and root elongation in Col wild-type and ataue1, ataue2, and ataue3 single mutants. (a) Seed germination assay. Surface-sterilized seeds of Col wild-type and the single mutants were plated on 1/2 MS plates with or without 1 μM ABA. Plates were kept at 4 °C in the dark for 2 days, then transferred to a growth room. Germinated seeds were counted every 12 h, and germination percentages were calculated. Data are presented as mean ± SD of three replicates. (b) Cotyledon greening assay. Surface-sterilized seeds were plated as above. Images were taken 10 days after transfer to the growth room. (c) Quantitative analysis of green seedlings. The number of seedlings with green cotyledons was counted, and percentages were calculated. Data are presented as mean ± SD of three replicates. (d) Root elongation assay. Surface-sterilized seeds were plated on 1/2 MS plates, kept at 4 °C in the dark for 2 days, then grown vertically for 3 days in a growth room. Seedlings were transferred to control plates or plates containing 5 μM and 10 μM ABA for 7 additional days before imaging. (e) Quantitative analysis of root elongation inhibition by ABA. Root lengths of new elongation were measured after imaging, and the percentage of inhibition was calculated for ABA concentrations of 5 μM (gray) and 10 μM (black). Data are presented as means ± SD of 11–16 seedlings.