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. 2024 Nov 22;13(23):3282. doi: 10.3390/plants13233282

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression of ABI5 in Col wild-type, 35S:AtAUE1 transgenic plants, and ataue123 triple mutants. Total RNA was isolated from ten-day-old seedlings of Col wild-type, 35S:AtAUE1 transgenic plants, and ataue123 triple mutants. cDNA was synthesized and used for qRT-PCR to assess ABI5 expression, with ACT2 serving as an internal control. ABI5 expression levels in Col wild-type seedlings were normalized to 1, and relative expression levels in 35S:AtAUE1 and ataue123 triple mutants were calculated accordingly. Data are presented as mean ± SD of three technical replicates. Significantly different from that of the Col wild-type (*** p < 0.001).